Download Advanced Techniques in Biological Electron Microscopy III by J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D. PDF

By J. Frank, M. Radermacher (auth.), James K. Koehler Ph. D. (eds.)

This quantity is a continuation of 2 previous books on complicated electron microscope strategies. the aim of this sequence has been to supply in­ intensity analyses of equipment that are thought of to be on the cutting edge of electron microscopic examine methods with functions within the organic sciences. The project of the current quantity is still that of a resource booklet for the examine practitioner or complex scholar, specifically one already good versed in uncomplicated electron optical tools. it's not intended to supply in­ troductory fabric, nor can this modest quantity wish to hide the complete spectrum of complicated expertise now on hand in electron microscopy. some time past decade, desktops have discovered their means into many study laboratories because of the big elevate in computing energy and stor­ age to be had at a modest fee. The ultrastructural sector has additionally benefited from this enlargement in a few methods in an effort to be illustrated during this quantity. 1/2 the contributions talk about applied sciences that both at once or in a roundabout way make huge use of machine methods.

Show description

Read or Download Advanced Techniques in Biological Electron Microscopy III PDF

Best atomic & nuclear physics books

History and Evolution of Concepts in Physics

Our knowing of nature, and specifically of physics and the legislation governing it, has replaced significantly because the days of the traditional Greek common philosophers. This ebook explains how and why those alterations happened, via landmark experiments in addition to theories that - for his or her time - have been innovative.

Hyperspherical Harmonics Expansion Techniques: Application to Problems in Physics

The ebook offers a generalized theoretical approach for fixing the fewbody Schrödinger equation. easy methods to unravel it when it comes to place vectors of constituent debris and utilizing commonplace mathematical options turn into too bulky and inconvenient whilst the approach comprises greater than debris.

Additional resources for Advanced Techniques in Biological Electron Microscopy III

Example text

In the maximum entropy method, among all the solutions compatible with the underdetermined equation system, the solution with the smallest information content, or the maximum entropy, is selected. 's results seem to indicate, the maximum entropy reconstruction is distinguished favorably from the ART Three-Dimensional Reconstruction of Nonperiodic Macromolecular Assemblies 25 reconstruction by a smaller amount of artifacts due to angular limitation. However, the great computational expense has thus far prevented a widespread use of this method.

1974; FRANK et ai. , 1986 b); the lateral views of the 40 S eukaryotic ribosomal subunit (BOUBLIK and HELLMAN 1978; FRANK et ai. 1981 b, 1982); and the top view of glutamine synthetase (VALENTINE et ai. 1968; FRANK et ai. 1978; see Figs. 4 and 5). , 1986). The azimuthal angles of the particles can be determined from an additional micrograph of the same specimen area at 0°, by employing rotational correlation techniques originally developed for the two-dimensional averaging of single particle projections (FRANK et ai.

As in other observations of an experimental quantity, the accuracy of estimating the sample mean is strongly enhanced if multiple measurements are available: the mean of each pixel is approximated by its average (5) The image P(Xi! Yk) resulting from averaging each pixel will be called average image. The signal-to-noise ratio of the average image (defined as the ratio standard deviation of signal: standard deviation of noise) improves with (FRANK et al. 1981 b). In order to interpret the averaged projection obtained by Eq.

Download PDF sample

Rated 4.27 of 5 – based on 21 votes