By Dominic M. Desiderio
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Extra info for Analysis of Neuropeptides by Liquid Chromatography and Mass Spectrometry
It was found t h a t intact neurointermediate Iobes chemicaIIy incorporated certain radiolabeled amino acids i n t o f o u r t o s i x forms of immunoreactive beta-endorphin. of those forms a r e compatible with a u t h e n t i c beta-endorphin, Four N-acetylated beta-endorphin , beta-endorphin 1-27, a n d N-acetylated beta-endorphinl-27. 3 two radiolabeled amino acids, methionine (35S) a n d tyrosine ( H ) , were The utilized i n t h i s s t u d y . Triethylamine-formic acid b u f f e r was utilized with acetonitrile a s organic modifier f o r a RP-HPLC g r a d i e n t .
T h e n e a r b y n e u r o n s , in t u r n , contain highly stereospecific r e c e p t o r s i t e s which a r e proteins embedded in t h e neuronal membrane a n d which bind those specific n e u r o t r a n s m i t t e r s which c r o s s t h e t r a n s - s y n a p t i c g a p . It h a s been hypothesized t h a t opiates e x e r t t h e i r role by inhibiting t h e firing r a t e of certain selected n e u r o n s . 2 . 5 NEUROREGULATORY PEPTIDES Neuroregulators a r e compounds which play a k e y role i n communication between a n d amongst n e r v e cells.
Most opiate actions a r e stereospecific and a r e produced almost entirely by the ( - ) isomer. A bioassay of opiates is based on the fact that opiates inhibit electrically-induced contractions of smooth muscle systems (guinea pig intestine). 4 NEURONS This section is necessarily brief for the purposes of this book. 1. Neurons a r e specialized cells that transmit information from one p a r t of a biologic system to a distantly located other p a r t and consist of a cell body which contains both dendrites and a nucleus, connected by a long portion called an axon.